Hands-on LC-MS for Metabolic Profiling
Dr Elizabeth Want
IIPTC Director and Senior Lecturer in Molecular Spectroscopy
This week long course aims to cover how to perform a metabolic profiling experiment, from start to finish. It will cover study design, sample preparation, the use of mass spectrometry for global profiling and targeted methodologies and data analysis.
Introductory lectures in mass spectrometry and chromatography, study design and sample preparation, followed by preparation of biological samples for analysis on subsequent days.
Days 2 and 3
Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions spent on each of the newest QToF instrumentation and the newest TQ instrumentation. Instrument set up, method development and acquisition will be covered. As we have set a maximum of 3 attendees per instrument this allows for hands-on participation by all. Day 3 finishes with introduction to data analysis.
Data analysis workshops where attendees will process the data acquired from the previous days, mixed with further statistics lectures, allowing for development of interpretation skills.
Application lectures, tips, tricks and troubleshooting, optional trip to the MRC-NIHR National Phenome Centre.
This course has been approved by the Royal Society of Chemistry. This event has been awarded 30 CPD credits by the Royal Society of Medicine in accordance with its current guidelines.